Accuris™ Taq High Fidelity HotStart DNA Polymerase, 25µl, PR1001-HFHS

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Accuris ScientificSKU: PR1001-HFHS-200

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Packaging: Case/200 PR1001-HFHS-200
Price:
Sale price$158.35 USD Regular price$186.30 USD

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Description

Accuris™ High Fidelity HotStart PR1001-HFHS DNA Polymerase is a ready-to-use 2X PCR master mix designed for highly accurate and reliable DNA amplification, especially in applications where sequence fidelity is critical such as cloning, mutagenesis, and sequencing.

This hot-start formulation combines a genetically engineered proofreading DNA polymerase with a proprietary antibody-mediated hot-start system. The enzyme remains inactive at room temperature, helping prevent non-specific amplification and primer-dimer formation until the initial denaturation step, improving specificity and reproducibility.

The polymerase includes 3’→5’ exonuclease proofreading activity, enabling high-fidelity DNA synthesis with significantly reduced error rates compared to standard Taq polymerase. It also delivers strong performance on difficult templates, including GC-rich and low-copy-number DNA, and is optimized for efficient amplification of targets up to approximately 10 kb

Applications:

  • Blunt End / TA Cloning
  • Colony PCR
  • Fast PCR
  • GC- & AT-Rich DNA Amplification
  • Low Copy PCR

Each mix includes:

  • High Fidelity Polymerase
  • Buffer [PCR Enhancers, 2mM dNTPs, 6mM MgCl2 , hot-start antibodies

Features:

  • Hot-start activation for improved specificity and reduced non-specific products
  • High fidelity DNA synthesis with proofreading activity
  • Optimized 2X master mix format for faster setup and fewer pipetting steps
  • Robust performance with complex, GC-rich, or low-quality templates
  • Suitable for cloning, site-directed mutagenesis, sequencing, and general high-accuracy PCR

Accuris™ HFHS HotStart Taq DNA Protocol   Accuris DNA Polymerase Catalog

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