Estimate shipping
Description
Accuris™ PR6000 SmartDye™ Terminator v3.1 Cycle Sequencing Kit is a direct, economical replacement for the Applied Biosystems™ BigDye™ Terminator v3.1 Cycle Sequencing Kit. Simply substitute SmartDye into your existing protocol — no protocol changes, no new mobility shift files, no recalibration and no revalidation are required.
Based on the proven Sanger method, SmartDye combines fluorescently labeled dideoxynucleotide terminators, DNA polymerase and an optimized 5X buffer to generate long, high-quality reads with a low error rate and high signal-to-noise ratio. It performs reliably on difficult templates, including GC-rich regions and GT repeats, and supports de novo sequencing, resequencing and finishing with PCR products, plasmids, BACs and fosmids. Extension takes just 3 minutes (2 minutes for many templates), and as little as 0.15 µl of mix can be used in a 5 µl reaction.
PR6000 kits ship overnight on dry ice. A flat $55.00 dry ice fee applies to the 24 and 100 reaction kits. Reagents cannot be returned for credit. For research use only.
Package Contents:
- All kits include SmartDye mix, 5X sequencing buffer, control and primer
- PR6000-24: 200 µl dye, 1 ml 5X buffer
- PR6000-100: 800 µl dye, 1 ml 5X buffer
- PR6000-1K: 4 x 2 ml dye, 12 ml 5X buffer
- PR6000-5K: 2 x 20 ml dye, 56 ml 5X buffer
- PR6000-25K: 10 x 20 ml dye, 466 ml 5X buffer
Features:
- Direct replacement for BigDye™ Terminator v3.1 — no protocol changes
- Long, high-quality reads with minimal noise
- Robust on GC-rich and GT-repeat templates
- Faster 3-minute extension
- High dilution factor — 0.15 µl mix per 5 µl reaction
- Store mix at -20°C to -80°C, protected from light
