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Description
Accuris™ PR2120-N qMAX Green One-Step RT-qPCR Kit (No ROX) combines first-strand cDNA synthesis and real-time PCR in a single tube. The kit pairs a ready-to-use 2X master mix with a companion 20X thermostable qMAX MMLV-derived Reverse Transcriptase blended with a potent RNase inhibitor, and is formulated for intercalating dye-based detection, with the option of a post-amplification melt curve to confirm product specificity.
Powered by Hot Start Taq DNA Polymerase and an optimized buffer chemistry, the mix delivers earlier quantification cycle (Ct) values and broad-range detection for greater sensitivity, speed and reproducibility. Specific RNA targets can be quantified from total RNA or mRNA with fewer pipetting steps and a shorter time to result — reverse transcription takes just 10 minutes at 50°C.
The No ROX formulation is intended for real-time instruments that do not require a passive reference dye, such as Bio-Rad CFX, Roche LightCycler, Qiagen Rotor-Gene and Agilent AriaMx systems. Recommended input is 10 pg to 100 ng total RNA (or as little as 0.01 pg mRNA) per 20 µl reaction. For research use only.
Applications:
- Gene expression analysis directly from RNA
- RNA virus detection and quantification
- Transcript quantification from total RNA or mRNA
- Melt curve analysis of amplification products
Package Contents:
- PR2120-N-100: 1 ml 2X reaction mix, 100 µl 20X RTase
- PR2120-N-500: 5 x 1 ml 2X reaction mix, 5 x 100 µl 20X RTase
- PR2120-N-1000: 10 x 1 ml 2X reaction mix, 10 x 100 µl 20X RTase
- PR2120-N-25BLK: 1 x 25 ml 2X reaction mix, 2 x 1.25 ml 20X RTase (2,500 reactions)
- PR2120-N-50BLK: 1 x 50 ml 2X reaction mix, 4 x 1.25 ml 20X RTase (5,000 reactions)
Features:
- One-step, single-tube reverse transcription and real-time PCR
- 2X master mix with Hot Start Taq DNA Polymerase and optimized buffer
- Thermostable qMAX MMLV-derived RTase with RNase inhibitor
- Earlier Ct values and broad dynamic range
- SYBR-type intercalating dye chemistry with melt curve capability
- No ROX formulation for instrument compatibility
- Store at -20°C; stable 12 months from receipt
