Accuris™ qMAX First Strand cDNA Synthesis Flex Kit, PR2110
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Accuris™ qMAX First Strand cDNA Synthesis Flex Kit, PR2110

Accuris™ qMAX First Strand cDNA Synthesis Flex Kit, PR2110

Save $16.93
Accuris ScientificSKU: PR2110-50
Price:
$95.93 USD $112.86 USD

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Factory new — sealed package

  • Shipped sealed in the original manufacturer packaging
  • Covered by the full manufacturer’s warranty
  • Never used, demonstrated, or previously sold
  • Drop ships from manufacturer. Subject to stock availability.
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Description

Accuris™ PR2110 qMAX First Strand cDNA Synthesis Flex Kit gives researchers full control over first-strand priming. Instead of a pre-blended buffer, the kit supplies separate solutions of oligo (dT) primers and random hexaprimers, so each reaction can be primed with oligo (dT), random hexamers, or a custom blend to suit the RNA source and downstream assay.

The kit is built around qMAX High Capacity Reverse Transcriptase, an exceptionally thermostable MMLV-derived enzyme combined with a potent RNase inhibitor. Transcription can be carried out at 42°C, or at 45°C for templates with heavy secondary structure, generating consistent, high yields of cDNA fragments up to 9 kb in length. The optimized 5X buffer contains dNTPs, MgCl2, stabilizers and enhancers, so no additives are needed.

The kit accepts a wide input range of 10 pg to 2 µg total RNA (or 5 pg to 0.5 µg mRNA) per 20 µl reaction. For research use only.

Applications:

  • First-strand cDNA synthesis for qPCR and endpoint RT-PCR
  • cDNA library construction and cloning of long transcripts
  • Gene expression studies from diverse RNA sources

Package Contents:

  • PR2110-50: 50 µl High Capacity RT (200 u/µl), 200 µl 5X RT Buffer, 25 µl Random Hexaprimers (20 µM), 25 µl Oligo (dT) Primers (20 µM)
  • PR2110-200: 4 x 50-reaction packs

Features:

  • Separate oligo (dT) and random hexamer primers for flexible priming
  • Input range of 10 pg to 2 µg total RNA
  • Thermostable, high-capacity MMLV-derived reverse transcriptase
  • Consistent, high yields of cDNA up to 9 kb
  • Optimized 5X buffer with dNTPs and MgCl2 — no additives required
  • Store at -20°C; stable 12 months from receipt

Product Documents

PDFProduct Insert | PDFProduct Catalog

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