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Description
Accuris™ PR2100-C qMAX cDNA Synthesis Kit quickly converts total RNA or mRNA into high-quality, qPCR-ready cDNA. The kit pairs a 5X reaction buffer pre-loaded with a balanced blend of anchored oligo (dT) primers and random hexamers with an exceptionally thermostable qMAX Reverse Transcriptase containing a potent RNase inhibitor, producing an unbiased cDNA population with true relative representation of every transcript.
Because the enzyme remains active at elevated temperatures, reverse transcription can be run at up to 55°C to relax RNA secondary structure in GC-rich templates (above 65% GC). The optimized buffer delivers high yields and reproducible data even from limited starting material and low-copy targets, and no additives are required. A standard reaction is complete in 30 minutes at 42°C followed by a 10-minute enzyme inactivation at 85°C.
Each 20 µl reaction produces enough cDNA for real-time PCR and other downstream applications. For research use only.
Applications:
- First-strand cDNA synthesis for qPCR and RT-PCR
- Gene expression analysis
- Low-copy and limited-sample transcript detection
- Templates with high secondary structure
Package Contents:
- PR2100-C-25: 25 µl 20X qMAX Reverse Transcriptase with RNase inhibitor, 100 µl 5X cDNA Synthesis Buffer with primers
- PR2100-C-100: 100 µl 20X RT (4 x 25 µl), 400 µl 5X buffer (4 x 100 µl)
- PR2100-C-250: 250 µl 20X RT (10 x 25 µl), 1 ml 5X buffer (10 x 100 µl)
Features:
- Unbiased, complete sequence representation from 4 pg to 0.5 µg total RNA
- Thermostable reverse transcriptase — reactions from 42°C up to 55°C
- Blend of anchored oligo (dT) and random hexamer primers included in the buffer
- Fast protocol: 30 minutes at 42°C plus 10 minutes at 85°C
- Consistent, high-yield cDNA from low-copy templates
- Store at -20°C; stable 12 months from receipt
