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Description
Accuris™ PR2001-L-100 qMAX Probe qPCR Mix (Low ROX) is a probe-based real-time PCR master mix optimized for use with TaqMan™, Scorpions®, and molecular beacon probes. Supplied as a ready-to-use 2X formulation, it delivers reliable quantification, superior sensitivity, and consistent performance across a wide range of qPCR applications.
The Accuris Hot Start Taq DNA Polymerase provides robust amplification across a variety of templates, including low copy number and difficult sequences, while the specially formulated buffer delivers earlier Ct values and broad dynamic range detection. Multiplex reactions can be performed without any loss in performance or decrease in detection sensitivity. The 2X mix requires little, if any, optimization and is compatible with both fast and standard cycling protocols.
Ideal for gene expression analysis, pathogen detection, genotyping, and multiplex assays on instruments requiring Low ROX normalization such as the Applied Biosystems StepOne™, StepOnePlus™, QuantStudio™ 3 and 5, and Bio-Rad CFX systems.
Applications:
- Gene Expression Analysis
- Pathogen Detection
- Genotyping Studies
- Multiplex qPCR Assays
- Fluorescent Detection of DNA/cDNA
Each Kit Includes:
- Accuris Hot Start Taq DNA Polymerase
- qMAX Probe optimized reaction buffer
- dNTPs
- Low ROX passive reference dye
- Optimized buffer for maximum efficiency & sensitivity
Features:
- Ready-to-use 2X Master Mix — compatible with fast and standard cycling protocols
- Low ROX formulation — optimized for instruments requiring passive reference dye normalization
- Hot-start enzyme system reduces non-specific amplification and primer-dimer formation
- Compatible with TaqMan™, Scorpions®, and molecular beacon probe chemistries
- Broad dynamic range with early Ct values
- Multiplex capable — no loss in performance or detection sensitivity
Accuris™ qMAX Probe Protocol Insert Accuris PCR Reagent Catalog