{"product_id":"accuris-qmax-probe-one-step-rt-qpcr-kit-no-rox-pr2121-n","title":"Accuris™ qMAX Probe One-Step RT-qPCR Kit, No ROX, PR2121-N","description":"\u003cp\u003eAccuris™ PR2121-N qMAX Probe One-Step RT-qPCR Kit (No ROX) combines first-strand cDNA synthesis and real-time PCR in a single tube. The kit pairs a ready-to-use 2X master mix with a companion 20X thermostable qMAX MMLV-derived Reverse Transcriptase blended with a potent RNase inhibitor, and is formulated for probe-based detection, including TaqMan®, Scorpions® and molecular beacon probes.\u003c\/p\u003e\n\u003cp\u003ePowered by Hot Start Taq DNA Polymerase and an optimized buffer chemistry, the mix delivers earlier quantification cycle (Ct) values and broad-range detection for greater sensitivity, speed and reproducibility. Specific RNA targets can be quantified from total RNA or mRNA with fewer pipetting steps and a shorter time to result — reverse transcription takes just 10 minutes at 50°C.\u003c\/p\u003e\n\u003cp\u003eThe No ROX formulation is intended for real-time instruments that do not require a passive reference dye, such as Bio-Rad CFX, Roche LightCycler, Qiagen Rotor-Gene and Agilent AriaMx systems. Recommended input is 10 pg to 100 ng total RNA (or as little as 0.01 pg mRNA) per 20 µl reaction. For research use only.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eGene expression analysis directly from RNA\u003c\/li\u003e\n\u003cli\u003eRNA virus detection and quantification\u003c\/li\u003e\n\u003cli\u003eTranscript quantification from total RNA or mRNA\u003c\/li\u003e\n\u003cli\u003eProbe-based and multiplex RT-qPCR assays\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003ePackage Contents:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003ePR2121-N-100: 1 ml 2X reaction mix, 100 µl 20X RTase\u003c\/li\u003e\n\u003cli\u003ePR2121-N-500: 5 x 1 ml 2X reaction mix, 5 x 100 µl 20X RTase\u003c\/li\u003e\n\u003cli\u003ePR2121-N-1000: 10 x 1 ml 2X reaction mix, 10 x 100 µl 20X RTase\u003c\/li\u003e\n\u003cli\u003ePR2121-N-25BLK: 1 x 25 ml 2X reaction mix, 2 x 1.25 ml 20X RTase (2,500 reactions)\u003c\/li\u003e\n\u003cli\u003ePR2121-N-50BLK: 1 x 50 ml 2X reaction mix, 4 x 1.25 ml 20X RTase (5,000 reactions)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eFeatures:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul style=\"margin-bottom:0\"\u003e\n\u003cli\u003eOne-step, single-tube reverse transcription and real-time PCR\u003c\/li\u003e\n\u003cli\u003e2X master mix with Hot Start Taq DNA Polymerase and optimized buffer\u003c\/li\u003e\n\u003cli\u003eThermostable qMAX MMLV-derived RTase with RNase inhibitor\u003c\/li\u003e\n\u003cli\u003eEarlier Ct values and broad dynamic range\u003c\/li\u003e\n\u003cli\u003eCompatible with TaqMan®, Scorpions® and molecular beacon probes\u003c\/li\u003e\n\u003cli\u003eNo ROX formulation for instrument compatibility\u003c\/li\u003e\n\u003cli\u003eStore at -20°C; stable 12 months from receipt\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003cdiv data-mla-docs=\"1\" style=\"border:1px solid #13275C;border-radius:6px;overflow:hidden;margin:14px 0 0\"\u003e\n\u003cp style=\"margin:0;padding:6px 14px;background:#13275C;color:#ffffff;font-weight:700;font-size:12px;letter-spacing:.08em;text-transform:uppercase\"\u003eProduct Documents\u003c\/p\u003e\n\u003cp style=\"margin:0;padding:12px 14px\"\u003e\u003cspan style=\"white-space:nowrap\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0606\/2669\/8378\/files\/Adobe_Icon_368009cd-f452-4bd7-b577-5709c393fd67.png?v=1782487710\" alt=\"PDF\" width=\"16\" height=\"16\" style=\"width:16px;height:16px;object-fit:contain;vertical-align:middle;margin-right:6px\"\u003e\u003ca href=\"https:\/\/accuris-usa.com\/wp-content\/uploads\/2021\/10\/PR2121-One-Step-Probe-qPCR-Product-Insert.pdf\" target=\"_blank\" rel=\"noopener\" title=\"Accuris qMAX Probe One-Step RT-qPCR Kit Product Insert\"\u003eProduct Insert\u003c\/a\u003e\u003c\/span\u003e  |  \u003cspan style=\"white-space:nowrap\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0606\/2669\/8378\/files\/Adobe_Icon_368009cd-f452-4bd7-b577-5709c393fd67.png?v=1782487710\" alt=\"PDF\" width=\"16\" height=\"16\" style=\"width:16px;height:16px;object-fit:contain;vertical-align:middle;margin-right:6px\"\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0606\/2669\/8378\/files\/PR1000-Series-Accuris-PCR-Reagents.pdf?v=1787962954\" target=\"_blank\" rel=\"noopener\" title=\"Accuris PCR Reagent Catalog - pdf\"\u003eProduct Catalog\u003c\/a\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Accuris Scientific","offers":[{"title":"Case\/100 PR2121-N-100","offer_id":72015581216906,"sku":"PR2121-N-100","price":160.55,"currency_code":"USD","in_stock":true},{"title":"Case\/500 PR2121-N-500","offer_id":72015581249674,"sku":"PR2121-N-500","price":698.13,"currency_code":"USD","in_stock":true},{"title":"Case\/1000 PR2121-N-1000","offer_id":72015581282442,"sku":"PR2121-N-1000","price":1279.91,"currency_code":"USD","in_stock":true},{"title":"Bulk\/2500 PR2121-N-25BLK","offer_id":72015581315210,"sku":"PR2121-N-25BLK","price":2466.83,"currency_code":"USD","in_stock":true},{"title":"Bulk\/5000 PR2121-N-50BLK","offer_id":72015581347978,"sku":"PR2121-N-50BLK","price":4379.88,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0606\/2669\/8378\/files\/Accuris-PR2121-qMAX-Probe-One-Step-RT-qPCR-Kit.jpg?v=1794443011","url":"https:\/\/medlabamerica.com\/products\/accuris-qmax-probe-one-step-rt-qpcr-kit-no-rox-pr2121-n","provider":"MedLabAmerica.com","version":"1.0","type":"link"}