{"product_id":"accuris-qmax-probe-one-step-rt-qpcr-kit-high-rox-pr2121-h","title":"Accuris™ qMAX Probe One-Step RT-qPCR Kit, High ROX, PR2121-H","description":"\u003cp\u003eAccuris™ PR2121-H qMAX Probe One-Step RT-qPCR Kit (High ROX) combines first-strand cDNA synthesis and real-time PCR in a single tube. The kit pairs a ready-to-use 2X master mix with a companion 20X thermostable qMAX MMLV-derived Reverse Transcriptase blended with a potent RNase inhibitor, and is formulated for probe-based detection, including TaqMan®, Scorpions® and molecular beacon probes.\u003c\/p\u003e\n\u003cp\u003ePowered by Hot Start Taq DNA Polymerase and an optimized buffer chemistry, the mix delivers earlier quantification cycle (Ct) values and broad-range detection for greater sensitivity, speed and reproducibility. Specific RNA targets can be quantified from total RNA or mRNA with fewer pipetting steps and a shorter time to result — reverse transcription takes just 10 minutes at 50°C.\u003c\/p\u003e\n\u003cp\u003eThe High ROX formulation is intended for real-time instruments that use a high level of ROX passive reference dye, such as Applied Biosystems 7000, 7300, 7700, 7900HT and StepOne systems. Recommended input is 10 pg to 100 ng total RNA (or as little as 0.01 pg mRNA) per 20 µl reaction. For research use only.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eGene expression analysis directly from RNA\u003c\/li\u003e\n\u003cli\u003eRNA virus detection and quantification\u003c\/li\u003e\n\u003cli\u003eTranscript quantification from total RNA or mRNA\u003c\/li\u003e\n\u003cli\u003eProbe-based and multiplex RT-qPCR assays\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003ePackage Contents:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003ePR2121-H-100: 1 ml 2X reaction mix, 100 µl 20X RTase\u003c\/li\u003e\n\u003cli\u003ePR2121-H-500: 5 x 1 ml 2X reaction mix, 5 x 100 µl 20X RTase\u003c\/li\u003e\n\u003cli\u003ePR2121-H-1000: 10 x 1 ml 2X reaction mix, 10 x 100 µl 20X RTase\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eFeatures:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul style=\"margin-bottom:0\"\u003e\n\u003cli\u003eOne-step, single-tube reverse transcription and real-time PCR\u003c\/li\u003e\n\u003cli\u003e2X master mix with Hot Start Taq DNA Polymerase and optimized buffer\u003c\/li\u003e\n\u003cli\u003eThermostable qMAX MMLV-derived RTase with RNase inhibitor\u003c\/li\u003e\n\u003cli\u003eEarlier Ct values and broad dynamic range\u003c\/li\u003e\n\u003cli\u003eCompatible with TaqMan®, Scorpions® and molecular beacon probes\u003c\/li\u003e\n\u003cli\u003eHigh ROX formulation for instrument compatibility\u003c\/li\u003e\n\u003cli\u003eStore at -20°C; stable 12 months from receipt\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003cdiv data-mla-docs=\"1\" style=\"border:1px solid #13275C;border-radius:6px;overflow:hidden;margin:14px 0 0\"\u003e\n\u003cp style=\"margin:0;padding:6px 14px;background:#13275C;color:#ffffff;font-weight:700;font-size:12px;letter-spacing:.08em;text-transform:uppercase\"\u003eProduct Documents\u003c\/p\u003e\n\u003cp style=\"margin:0;padding:12px 14px\"\u003e\u003cspan style=\"white-space:nowrap\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0606\/2669\/8378\/files\/Adobe_Icon_368009cd-f452-4bd7-b577-5709c393fd67.png?v=1782487710\" alt=\"PDF\" width=\"16\" height=\"16\" style=\"width:16px;height:16px;object-fit:contain;vertical-align:middle;margin-right:6px\"\u003e\u003ca href=\"https:\/\/accuris-usa.com\/wp-content\/uploads\/2021\/10\/PR2121-One-Step-Probe-qPCR-Product-Insert.pdf\" target=\"_blank\" rel=\"noopener\" title=\"Accuris qMAX Probe One-Step RT-qPCR Kit Product Insert\"\u003eProduct Insert\u003c\/a\u003e\u003c\/span\u003e  |  \u003cspan style=\"white-space:nowrap\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0606\/2669\/8378\/files\/Adobe_Icon_368009cd-f452-4bd7-b577-5709c393fd67.png?v=1782487710\" alt=\"PDF\" width=\"16\" height=\"16\" style=\"width:16px;height:16px;object-fit:contain;vertical-align:middle;margin-right:6px\"\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0606\/2669\/8378\/files\/PR1000-Series-Accuris-PCR-Reagents.pdf?v=1787962954\" target=\"_blank\" rel=\"noopener\" title=\"Accuris PCR Reagent Catalog - pdf\"\u003eProduct Catalog\u003c\/a\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Accuris Scientific","offers":[{"title":"Case\/100 PR2121-H-100","offer_id":72015582167178,"sku":"PR2121-H-100","price":160.55,"currency_code":"USD","in_stock":true},{"title":"Case\/500 PR2121-H-500","offer_id":72015582199946,"sku":"PR2121-H-500","price":698.13,"currency_code":"USD","in_stock":true},{"title":"Case\/1000 PR2121-H-1000","offer_id":72015582232714,"sku":"PR2121-H-1000","price":1279.91,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0606\/2669\/8378\/files\/Accuris-PR2121-qMAX-Probe-One-Step-RT-qPCR-Kit.jpg?v=1794443011","url":"https:\/\/medlabamerica.com\/products\/accuris-qmax-probe-one-step-rt-qpcr-kit-high-rox-pr2121-h","provider":"MedLabAmerica.com","version":"1.0","type":"link"}