{"product_id":"accuris-qmax-first-strand-cdna-synthesis-flex-kit-pr2110","title":"Accuris™ qMAX First Strand cDNA Synthesis Flex Kit, PR2110","description":"\u003cp\u003eAccuris™ PR2110 qMAX First Strand cDNA Synthesis Flex Kit gives researchers full control over first-strand priming. Instead of a pre-blended buffer, the kit supplies separate solutions of oligo (dT) primers and random hexaprimers, so each reaction can be primed with oligo (dT), random hexamers, or a custom blend to suit the RNA source and downstream assay.\u003c\/p\u003e\n\u003cp\u003eThe kit is built around qMAX High Capacity Reverse Transcriptase, an exceptionally thermostable MMLV-derived enzyme combined with a potent RNase inhibitor. Transcription can be carried out at 42°C, or at 45°C for templates with heavy secondary structure, generating consistent, high yields of cDNA fragments up to 9 kb in length. The optimized 5X buffer contains dNTPs, MgCl\u003csub\u003e2\u003c\/sub\u003e, stabilizers and enhancers, so no additives are needed.\u003c\/p\u003e\n\u003cp\u003eThe kit accepts a wide input range of 10 pg to 2 µg total RNA (or 5 pg to 0.5 µg mRNA) per 20 µl reaction. For research use only.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eFirst-strand cDNA synthesis for qPCR and endpoint RT-PCR\u003c\/li\u003e\n\u003cli\u003ecDNA library construction and cloning of long transcripts\u003c\/li\u003e\n\u003cli\u003eGene expression studies from diverse RNA sources\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003ePackage Contents:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003ePR2110-50: 50 µl High Capacity RT (200 u\/µl), 200 µl 5X RT Buffer, 25 µl Random Hexaprimers (20 µM), 25 µl Oligo (dT) Primers (20 µM)\u003c\/li\u003e\n\u003cli\u003ePR2110-200: 4 x 50-reaction packs\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eFeatures:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul style=\"margin-bottom:0\"\u003e\n\u003cli\u003eSeparate oligo (dT) and random hexamer primers for flexible priming\u003c\/li\u003e\n\u003cli\u003eInput range of 10 pg to 2 µg total RNA\u003c\/li\u003e\n\u003cli\u003eThermostable, high-capacity MMLV-derived reverse transcriptase\u003c\/li\u003e\n\u003cli\u003eConsistent, high yields of cDNA up to 9 kb\u003c\/li\u003e\n\u003cli\u003eOptimized 5X buffer with dNTPs and MgCl\u003csub\u003e2\u003c\/sub\u003e — no additives required\u003c\/li\u003e\n\u003cli\u003eStore at -20°C; stable 12 months from receipt\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003cdiv data-mla-docs=\"1\" style=\"border:1px solid #13275C;border-radius:6px;overflow:hidden;margin:14px 0 0\"\u003e\n\u003cp style=\"margin:0;padding:6px 14px;background:#13275C;color:#ffffff;font-weight:700;font-size:12px;letter-spacing:.08em;text-transform:uppercase\"\u003eProduct Documents\u003c\/p\u003e\n\u003cp style=\"margin:0;padding:12px 14px\"\u003e\u003cspan style=\"white-space:nowrap\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0606\/2669\/8378\/files\/Adobe_Icon_368009cd-f452-4bd7-b577-5709c393fd67.png?v=1782487710\" alt=\"PDF\" width=\"16\" height=\"16\" style=\"width:16px;height:16px;object-fit:contain;vertical-align:middle;margin-right:6px\"\u003e\u003ca href=\"https:\/\/accuris-usa.com\/wp-content\/uploads\/2023\/02\/PR2110-1st-Strand-cDNA-Synthesis-Flex-Kit.pdf\" target=\"_blank\" rel=\"noopener\" title=\"Accuris qMAX First Strand cDNA Synthesis Flex Kit Product Insert\"\u003eProduct Insert\u003c\/a\u003e\u003c\/span\u003e  |  \u003cspan style=\"white-space:nowrap\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0606\/2669\/8378\/files\/Adobe_Icon_368009cd-f452-4bd7-b577-5709c393fd67.png?v=1782487710\" alt=\"PDF\" width=\"16\" height=\"16\" style=\"width:16px;height:16px;object-fit:contain;vertical-align:middle;margin-right:6px\"\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0606\/2669\/8378\/files\/PR1000-Series-Accuris-PCR-Reagents.pdf?v=1787962954\" target=\"_blank\" rel=\"noopener\" title=\"Accuris PCR Reagent Catalog - pdf\"\u003eProduct Catalog\u003c\/a\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Accuris Scientific","offers":[{"title":"Case\/50 PR2110-50","offer_id":72015569354890,"sku":"PR2110-50","price":95.93,"currency_code":"USD","in_stock":true},{"title":"Case\/200 PR2110-200","offer_id":72015569387658,"sku":"PR2110-200","price":386.73,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0606\/2669\/8378\/files\/Accuris-PR2110-qMAX-First-Strand-cDNA-Synthesis-Flex-Kit.jpg?v=1794443011","url":"https:\/\/medlabamerica.com\/products\/accuris-qmax-first-strand-cdna-synthesis-flex-kit-pr2110","provider":"MedLabAmerica.com","version":"1.0","type":"link"}