{"product_id":"accuris-qmax-cdna-synthesis-kit-pr2100-c","title":"Accuris™ qMAX cDNA Synthesis Kit, PR2100-C","description":"\u003cp\u003eAccuris™ PR2100-C qMAX cDNA Synthesis Kit quickly converts total RNA or mRNA into high-quality, qPCR-ready cDNA. The kit pairs a 5X reaction buffer pre-loaded with a balanced blend of anchored oligo (dT) primers and random hexamers with an exceptionally thermostable qMAX Reverse Transcriptase containing a potent RNase inhibitor, producing an unbiased cDNA population with true relative representation of every transcript.\u003c\/p\u003e\n\u003cp\u003eBecause the enzyme remains active at elevated temperatures, reverse transcription can be run at up to 55°C to relax RNA secondary structure in GC-rich templates (above 65% GC). The optimized buffer delivers high yields and reproducible data even from limited starting material and low-copy targets, and no additives are required. A standard reaction is complete in 30 minutes at 42°C followed by a 10-minute enzyme inactivation at 85°C.\u003c\/p\u003e\n\u003cp\u003eEach 20 µl reaction produces enough cDNA for real-time PCR and other downstream applications. For research use only.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eFirst-strand cDNA synthesis for qPCR and RT-PCR\u003c\/li\u003e\n\u003cli\u003eGene expression analysis\u003c\/li\u003e\n\u003cli\u003eLow-copy and limited-sample transcript detection\u003c\/li\u003e\n\u003cli\u003eTemplates with high secondary structure\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003ePackage Contents:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003ePR2100-C-25: 25 µl 20X qMAX Reverse Transcriptase with RNase inhibitor, 100 µl 5X cDNA Synthesis Buffer with primers\u003c\/li\u003e\n\u003cli\u003ePR2100-C-100: 100 µl 20X RT (4 x 25 µl), 400 µl 5X buffer (4 x 100 µl)\u003c\/li\u003e\n\u003cli\u003ePR2100-C-250: 250 µl 20X RT (10 x 25 µl), 1 ml 5X buffer (10 x 100 µl)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eFeatures:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul style=\"margin-bottom:0\"\u003e\n\u003cli\u003eUnbiased, complete sequence representation from 4 pg to 0.5 µg total RNA\u003c\/li\u003e\n\u003cli\u003eThermostable reverse transcriptase — reactions from 42°C up to 55°C\u003c\/li\u003e\n\u003cli\u003eBlend of anchored oligo (dT) and random hexamer primers included in the buffer\u003c\/li\u003e\n\u003cli\u003eFast protocol: 30 minutes at 42°C plus 10 minutes at 85°C\u003c\/li\u003e\n\u003cli\u003eConsistent, high-yield cDNA from low-copy templates\u003c\/li\u003e\n\u003cli\u003eStore at -20°C; stable 12 months from receipt\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003cdiv data-mla-docs=\"1\" style=\"border:1px solid #13275C;border-radius:6px;overflow:hidden;margin:14px 0 0\"\u003e\n\u003cp style=\"margin:0;padding:6px 14px;background:#13275C;color:#ffffff;font-weight:700;font-size:12px;letter-spacing:.08em;text-transform:uppercase\"\u003eProduct Documents\u003c\/p\u003e\n\u003cp style=\"margin:0;padding:12px 14px\"\u003e\u003cspan style=\"white-space:nowrap\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0606\/2669\/8378\/files\/Adobe_Icon_368009cd-f452-4bd7-b577-5709c393fd67.png?v=1782487710\" alt=\"PDF\" width=\"16\" height=\"16\" style=\"width:16px;height:16px;object-fit:contain;vertical-align:middle;margin-right:6px\"\u003e\u003ca href=\"https:\/\/accuris-usa.com\/wp-content\/uploads\/2020\/10\/PR2100-C-Accuris-cDNA-Synthesis-Kit.pdf\" target=\"_blank\" rel=\"noopener\" title=\"Accuris qMAX cDNA Synthesis Kit Product Insert\"\u003eProduct Insert\u003c\/a\u003e\u003c\/span\u003e  |  \u003cspan style=\"white-space:nowrap\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0606\/2669\/8378\/files\/Adobe_Icon_368009cd-f452-4bd7-b577-5709c393fd67.png?v=1782487710\" alt=\"PDF\" width=\"16\" height=\"16\" style=\"width:16px;height:16px;object-fit:contain;vertical-align:middle;margin-right:6px\"\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0606\/2669\/8378\/files\/PR1000-Series-Accuris-PCR-Reagents.pdf?v=1787962954\" target=\"_blank\" rel=\"noopener\" title=\"Accuris PCR Reagent Catalog - pdf\"\u003eProduct Catalog\u003c\/a\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Accuris Scientific","offers":[{"title":"Case\/25 PR2100-C-25","offer_id":72015561293962,"sku":"PR2100-C-25","price":112.04,"currency_code":"USD","in_stock":true},{"title":"Case\/100 PR2100-C-100","offer_id":72015561326730,"sku":"PR2100-C-100","price":394.33,"currency_code":"USD","in_stock":true},{"title":"Case\/250 PR2100-C-250","offer_id":72015561359498,"sku":"PR2100-C-250","price":935.11,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0606\/2669\/8378\/files\/Accuris-PR2100-C-qMAX-cDNA-Synthesis-Kit.jpg?v=1794443011","url":"https:\/\/medlabamerica.com\/products\/accuris-qmax-cdna-synthesis-kit-pr2100-c","provider":"MedLabAmerica.com","version":"1.0","type":"link"}